Volume 18 Part 1 Article 98: Microbial Community Diversity and Structure Analysis of Casing Soil During Mushroom (Agaricus bisporus) Growth

Volume 18 Part 1 Article 98
Year 2012
Title: Microbial Community Diversity and Structure Analysis of Casing Soil During Mushroom (Agaricus bisporus) Growth
Authors: Weiming Cai, Qunli Jin, Weilin Feng, Huaiying Yao and Yueyan Liu

Abstract:

The culturable bacterial population, phospholipid fatty acid (PLFA) profile and PCR-DGGE of casing soil were investigated at different mushroom (Agaricus bisporus) cropping stages. The change in soil bacterial PLFAs was always accompanied by a change in the soil culturable bacterial population in the first flush. Comparatively higher bacterial PLFAs and culturable bacterial populations were found in casing soil at the primordia formation stage of the first flush. There was a significant increase in the ratio of fungal to bacterial PLFAs during mushroom growth. Multivariate analysis of PLFA data demonstrated that the mushroom cropping stage could considerably affect the microbial community structure of casing soil. The bacterial population increased significantly from casing soil application to the primordia formation stage of the first flush. Casing soil application resulted in an increase in the ratio of gram-negative bacterial PLFAs to gram-positive bacterial PLFAs, which may suggest that some gram-negative bacteria play an important role in mushroom sporophore initiation. PCR-DGGE of different casing soils showed that the amount of bands in the DGGE map was increased and the strength was enhanced as the mushroom growth. It suggested that the genetic diversity and structure of the bacterial community were affected by the mushroom and these were consistent with the results obtained from the PLFA assays. Based on the observation from the DGGE map of the casing soils, on which the mushroom had high output, the propagation of some bacterial communities which were represented by three special bands was initiated and the result probably signified a choice symbiotic interaction between the mushroom and these bacteria.

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