Volume 18 Part 1 Article 63
Title: First Report of a Laccase from Genus Abortiporus with Anti-Proliferative Activity Against Tumor Cells
Authors: Guo-Qing Zhang, Tian Tian, Yue-Ping Liu, He-Xiang Wang and Qing-Jun Chen
A laccase from the white rot fungus Abortiporus biennis was purified and characterized with a purification scheme involving ammonium sulfate saturation, ion exchange chromatography on DEAE-cellulose, CM-cellulose and Q-Sepharose, and followed by fast protein liquid chromatography-gel filtration on Superdex 75. The molecular mass of the enzyme was 56 kDa as estimated by SDS-PAGE. The protein was monomeric with an N-terminal amino acid sequence of AIGPVADLTISNG, which exhibited partial sequence homology to laccases from family Polyporaceae, but was different from other families. It had an optimum pH of 3.0-4.0, and an optimum temperature of 60°C. It displayed a Km value of 33.5 μM using ABTS as a substrate at pH 3.5 and 37°C. The laccase possessed anti-proliferative activity against Hep G2 and MCF-7 tumor cells with IC50 values of 12.5 μM and 6.7 μM, respectively, and exhibited inhibitory activity against HIV-1 RT with an IC50 of 9.2 μM.Please login to download the PDF for this proceeding.