Volume 5 Part 1 Article 20: Observations on Multisporous Isolates from the Cultivated Mushroom, Agaricus bisporus (Lange) Sing.

Volume 5 Part 1 Article 20
Year 1963
Title: Observations on Multisporous Isolates from the Cultivated Mushroom, Agaricus bisporus (Lange) Sing.
Authors: C.W. Bretzloff, W.A. Robbins, and J.H. Curme

Abstract:

Grain spawn of a white strain was purchased from a spawn supplier for commercial mushroom production. From the first break, five mushrooms were selected and multisporous spore collections made from each. These collections were designated A to E. Spores from the collections were germinated on potato dextrose yeast extract agar (PDYA) plates. Stimulation was supplied via the presence of an older colony at one side of the plate. This stimulatory colony was derived by tissue culture from the same strain. Subsequent multisporous transfers were made to the same medium in agar slants. From these cultures, transfers were made to PDYA in test tubes or petri dishes and to 250 ml flasks of rye grain. These cultures were used for inoculating liter flasks of rye grain which were used for spawning experimental plots.

The original colonies following spore germination appeared normal. The first transfers to agar slants developed normal colonies but the collections A and D stained the medium brownish. By the second or third transfers on PDYA, differences among the collections had developed in colony growth rate, color and type. This was contrary to experience with cultures prepared in a similar manner from another strain, the 70-2 cream mushroom.

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