Volume 16 Part 1 Article 71: Response of Trichoderma and Pleurotus to Extracts of Cypress Leaves

Volume 16 Part 1 Article 71
Year 2004
Title: Response of Trichoderma and Pleurotus to Extracts of Cypress Leaves
Authors: J.K. Yang, B.K. Lim, G. Alm and D.L. Rinker


Chemical treatments are often the most effective measure for managing green mold (Trichoderma). However, the development of safe, natural, biodegradable compounds that inhibit or kill green mold with minimal danger to the consumer is desirable. Extracts from plant leaves were evaluated for activity against Trichoderma and Pleurotus in an effort to identify alternative and safe compounds for managing mushroom pests. The extracts from leaves of Chamaecyparis obtusa (hinoki cypress) and C. pisifera (sawara cypress) were isolated by distillation. Extracts of hinoki cypress consisted mainly of a-pinene, limonene, bornyl acetate, terpinyl acetate and elemol. The major compounds of sawara cypress extracts were a-pinene, mycerene, limonene and isobornyl acetate. Extracts of leaves from two Chamaecyparis species were tested at 50, 100, 500, 1,000 and 2,000 ppm for antifungal activity against Trichoderma and Pleurotus. Differences in growth inhibition between the extracts of hinoki and sawara leaves against Trichoderma were observed. Hinoki leaf extract incorporated in potato dextrose agar at 500 ppm provided antifungal activity against Trichoderma. Growth of P. ostreatus and P. florida was less affected by the leaf extract than that of the Trichoderma species evaluated.

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