Volume 16 Part 1 Article 7
Title: Expression Profiles of Key Genes in Agaricus bisporus Postharvest Development of Fruiting Bodies
Authors: D.C. Eastwood, M.J. Sergeant, A. Mead and K.S. Burton
Postharvest quality loss is a major factor affecting the mushroom Agaricus bisporus, especially when sold fresh and unprocessed. Following harvest, the mushroom continues to develop leading to characteristics viewed negatively by consumers. These include loss of texture and firmness, increased bruising, darkening in color and, ultimately, senescence. The mushroom is able to maintain cap expansion, and spore production and release despite being nutritionally isolated from the mycelium. Differential screening has identified 20 gene families upregulated during postharvest storage. The function of these genes included stress tolerance, polymer breakdown, nitrogen metabolism, cell wall growth and DNA binding. The up-regulated nature of the genes with diverse physiological roles suggests that regulation of postharvest change is controlled at the molecular level. By studying the temporal and spatial expression profiles of key postharvest genes, it should be possible to identify control mechanisms relating to detachment and development following harvest. These include the switches turning the genes on, longterm regulation through development into senescence and differences existing among the tissue types.Please login to download the PDF for this proceeding.