Volume 16 Part 1 Article 61: Investigation of the Interaction Between Verticillium fungicola and Agaricus bisporus

Volume 16 Part 1 Article 61
Year 2004
Title: Investigation of the Interaction Between Verticillium fungicola and Agaricus bisporus
Authors: G.D. Foster, R. Amey, A. Bailey and P.R. Mills

Abstract:

Pathogenic threats to Agaricus include bacteria, viruses, mites, insects and fungi, with the most significant to the commercial industry being Verticillium fungicola. Verticillium infection is manifested in three types of symptoms (dependent on time of infection), designated spotty cap, stipe blowout and dry bubble, all of which can drastically reduce yields and value of the crop. An aim of our research was to develop molecular tools for Verticillium that will allow us to study the interaction between Verticillium and Agaricus. These tools included transformation methods, marker gene techniques (GUS, GFP) as well as gene-knockout technologies. This has involved the use of Agrobacterium and T-DNA to introduce disruption constructs into V. fungicola as part of a molecular investigation into the interaction between Verticillium and A. bisporus. We have developed an efficient transformation system for Verticillium that we have now adapted to give high levels of targeted mutagenesis. We have used this technology to identify and investigate genes involved in the infection and pathogenicity of Verticillium on Agaricus.

Please login to download the PDF for this proceeding.