Volume 16 Part 1 Article 22: Development of a New Strain by Backcross Mating and Molecular Marker-assisted Selection in Flammulina velutipes

Volume 16 Part 1 Article 22
Year 2004
Title: Development of a New Strain by Backcross Mating and Molecular Marker-assisted Selection in Flammulina velutipes
Authors: W.S. Kong, Y.H. Cho, C.S. Jhune, Y.B. Yoo and K.H. Kim

Abstract:

The winter mushroom, Flammulina velutipes, is grown commercially in Korea by a mechanized bottle cultivation system under controlled environmental conditions. The fruiting body of the wild type F. velutipes is characterized by a brown pileus and dark brown stipe. However, brown fruiting bodies are not popular in Korea, as consumers prefer a white colored product. The objective of our research was to breed new strains of F. velutipes having white fruiting bodies. Molecular markers linked to fruiting body color were identified using random amplification of polymorphic DNA. Bulked segregant analysis was performed to backcross lines for fruiting body color. At the same time, we were interested in developing a strain that could be grown at an elevated temperature, because of the high-energy cost associated with summertime production. Of 74 dikaryotic isolates tested, three brown isolates were selected based on their ability to grow at an elevated temperature. A molecular marker-assisted backcrossing scheme was designed for the development of a new mid-temperature white strain of F. velutipes.

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