Volume 16 Part 1 Article 11
Title: Cloning and Sequence Analysis of the Glyceraldehyde-3-Phosphate Dehydrogenase Gene in Flammulina velutipes
Authors: C.Y. Kuo, S.Y. Chuo, C.H. Ling, C.S. Chen, R.S. Hseu and C.T. Huang
The glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of Flammulina velutipes was isolated, and the complete gpd sequence (from ATG to TAA) of the gene proved to be 1,489 bp and contained nine introns of similar size. The locations of the nine introns were similar to those for other basidiomycetes, which may reflect the evolutionary divergence of these mushrooms. The position of only one intron was conserved between basidiomycetes and ascomycetes, suggesting that there exists a clear boundary between ascomycetes and basidiomycetes with respect to intron positioning within the gpd genes. The F. velutipes gpd gene encoded a protein of 339 amino acids and its putative amino acid sequence revealed a high similarity to an analogous protein derived from other basidiomycetes, especially Lentinula edodes, with a similarity of 83%. The active residue cysteine was located at the position 151. Results of Southern blot analyses suggested that there existed only one copy of the gpd gene in the genome of F. velutipes, and that there was one typical TATA box and two CAAT boxes located in the 5’-flanking region. The TATA box was located 83 bp upstream of the start codon, while the CAAT boxes were 669 and 719 bp upstream of the initiating ATG. The CAAT boxes were farther from the initiating ATG than was the case for those found in various filamentous fungi. The transcription start point was located in a CT stretch, 53 bp upstream of the start codon. As for other basidiomycetes, a gpd box, pgk box, qut box, or qa box was not found.Please login to download the PDF for this proceeding.