Volume 13 Part 1 Article 55
Title: The occurrence of ascocarps of Diehliomyces microsporus, the cause of False Truffle disease
Authors: M.W. Wood and X.T. Fletcher
Mycelial disks of Diehliomyces microsporus were used to inoculate spawned mushroom compost which was incubated at a range of temperatures. Ascocarps formed most extensively at 25°C and 30°C and not at 15°C or 37°C. Laccase activity, an indirect quantitative measure of the development of A. Bisporus, indicated best development at 22°C in the abscence of D. microsporus but in the presence of D. microsporus activity was considerably reduced at both 22°C and 25°C. Following compost inoculation and incubation at 30°C for 0.25 – 2 days, treatments were returned to 22° or 15°C. At 22°C most ascocarps developed following initial incubation at 30oC for 1.25 – 2 days but incubation at 15°C resulted in no ascocarp formation even after 2 days incubation at 30°C. When ascospores of D. microsporus were used as inoculum, ascocarps did not develop when the boxes were not cased. The reason for this was not determined. Following treatment of ascospore suspensions at 40°C-80°C for 10 minutes, viability was tested by inoculating spawned compost. At temperatures in excess of 60°C ascocarps were not formed. Varying times of treatment of ascospores at 60°C, established that a treatment time of 2 hours was necessary to prevent ascocarp formation. Pseudomonas putida was the dominant bacterium isolated from decaying ascocarps.Please login to download the PDF for this proceeding.