Volume 13 Part 1 Article 38
Title: Alteration of laccases and acid phosphatase in mono- and dikaryotic mycelia of Lentinus edodes
Authors: S. Panichajakul, W. Yindeeyoungyeon and S. Triratana
The time course changes of laccase (EC.18.104.22.168) and acid phosphatase (EC.22.214.171.124) activities and isozyme patterns were investigated to elucidate the possible roles in fruiting for Lentinus edodes. The intra- and extra- cellular enzymes were determined in mono- and di-karyons of parental and hybrid mycelium growing in liquid culture. Only one type of acid phosphatase could be detected during the entire growth period on PDY (potato dextrose yeast extract). Several electrophoretically distinguishable forms of laccase were established. The enzyme activity and isozyme number which were measuring in the mono- karyons illustrated to be lowered than the dikaryon parents and hybrids. The growth associated pattern of acid phosphatase and laccase were found in both types of mycelia. Results fron the electrophoretic profiles of laccase also indicated partly genetic inheritance of a l l the isozyme forms in some hybrids from the parents. Most of the hybrid cultures which provided good fruiting produced significantly elevated level of laccase when cultivated in liquid medium, while there was inconsistent difference in acid phosphatase from the mycelium.Please login to download the PDF for this proceeding.