Volume 13 Part 1 Article 36
Title: Molecular biology of edible fungi
Authors: M.P. Challen, B.G. Rao and T.J. Elliott
Molecular biology has become increasingly pervasive in studies of the fungi but as yet has had little impact on the edible species. The potential of RFLPs, transformation, and related technologies to this important group of fungi will be reviewed. Most effort has been directed at the major cultivated species Agaricus bisporus and programmes are in place to exploit RFLPs in Agaricus breeding and to develop transformation. No effective positively selectable marker is available for transforming this group of fungi. We are attempting to develop an effective system for this group based on selection for resistance to the fungicide carboxin. This fungicide was developed for the control of rusts and smuts and has been shown to be particularly effective against the basidiomycetes. A dominant mutation to carboxin resistance which segregates as a single gene has been identified in the ink-cap fungus Coprinus bilanatus. A cosmid library has been made from this mutant and is currently being screened for the carboxin clone by heterologous transformation of Coprinus cinereus. This and other strategies are discussed.Please login to download the PDF for this proceeding.