Volume 4 Part 1 Article 6: Growing of Monosporous Strains of Psalliota Campestris (Cultivated Form).

Volume 4 Part 1 Article 6
Year 1960
Title: Growing of Monosporous Strains of Psalliota Campestris (Cultivated Form).
Authors: G. Borzini and J. Ceruti Scurti

Abstract:

At the Paris congress we have communicated the methods employed by us to obtain monosporous strains of Psalliota campestns (cultivated form), as the germination of spores of Psalliota arvensis did not succeed.

With the methods described (micromanipulation, dilution and Petri dishes with little glass discs in them, described elsewhere (1,2) we have made more than 600 monosporous cultures, starting from spores of the same fruit body. We have however obtained only six well developed monosporous strains. The mycelia of these, transplanted in Petri dishes, showed some differences, which we have illustrated in the previous note (1). Indeed some cultures showed mycelial ropes at the agar surface, others, on the contrary, formed abundant white mycelium, with many aereal hyphae.

In order to ascertain whether the differences among the mycelia of various strains, in relation also to plurisporous strains, were also transmissible to the sporophores, we have made essay in pots and essays in mushroom caves. For pot essays, 1 liter milk bottles have been employed, using the technique illustrated by Borzini (1949) and described at the Paris congress (1956) (1). Some bottles were filled with a mixture of straw, oats, hay and sand, adding a mineral salts solution, others were filled with washed horse manure.

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