Volume 17 Part 1 Article 9: Study on the Expression of Cold-Induced Cor3 Gene from Volvariella volvacea by Real-Time Quantitative PCR

Volume 17 Part 1 Article 9
Year 2008
Title: Study on the Expression of Cold-Induced Cor3 Gene from Volvariella volvacea by Real-Time Quantitative PCR
Authors: Hong Wang, and Ming-Jie Chen

Abstract:

Based on Cor3 gene of Volvariella volvacea obtained from cold-induced cDNA bank from V. volvacea by mRNA differential display technology followed by library screening, after the total RNA of V. volvacea mycelia was extracted and amplified by RT–PCR, the plasmids containing Cor3 gene (target gene) and GPD gene (internal control gene) were constructed and diluted serially. The standard curves of Cor3 gene and GPD gene of V. volvacea for real-time quantitative PCR were then made. Expression of cold-induced Cor3 gene from V. volvacea at low temperature was studied by real-time quantitative PCR, in an initial study on the function of Cor3 gene in metabolisation and autolysation of V. volvacea at low temperature.

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