Volume 17 Part 1 Article 67: Bioaccumulation of Heavy Metals and Enzyme Activities of Pleurotus ostreatus

Volume 17 Part 1 Article 67
Year 2008
Title: Bioaccumulation of Heavy Metals and Enzyme Activities of Pleurotus ostreatus
Authors: C.O. Adenipekun and O.M. Omoruyi


Soils collected from cement, black-oil and battery-waste-polluted sites were incubated with Pleurotus ostreatus for periods of 1 month and 2 months. Contents of heavy metals – lead, copper, manganese, iron and nickel – were taken in each soil sample after 1 and 2 months of incubation. The control experiment included all the soil samples not inoculated with the fungus. In the cement-polluted soil, there was a reduction in lead from 8.00 ppm in the control sample to 6.00 ppm after 1 month, and after 2 months, the lead content was still 6.00 ppm. A similar result was observed in nickel which fell from 0.40 ppm to 0.05 ppm after 1 month and did not change 2 two months. In black-oil-polluted soil, after each incubation period, the values of lead, copper manganese and nickel reduced, while in battery-waste-polluted soil, the copper and manganese content decreased as the incubation period increased, while there was a decrease followed by an increase in lead, iron and nickel content of the soil sample. In each case, there was a significant difference in the values of the control and the value of the incubated soil samples at (p ≤ 0.05). The result of the enzyme activities showed a decrease in peroxidase activity, from 42 to 39, in black-oil-polluted soil, while an increase was observed in the other soil samples with increase in incubation period from 27 to 43 for cement-polluted soil and from 18 to 27 activity/minute in battery-waste-polluted soil. The highest polyphenol oxidase activity was also observed in black-oil polluted soil (62), while the lowest was recorded in cement-polluted soil (21 activity/minute) after 1 month in each case. The statistical analysis showed that increase in incubation period resulted in decrease in heavy metal content. The study thus shows that P. ostreatus has the ability to accumulate heavy metals, related to its ability to produce enzymes.

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