Volume 17 Part 1 Article 55: Solid-State and Submerged Cultivation of Grifola frondosa Polysaccharides

Volume 17 Part 1 Article 55
Year 2008
Title: Solid-State and Submerged Cultivation of Grifola frondosa Polysaccharides
Authors: M. Berovič, B. Boh and B. Wraber

Abstract:

Original Grifola frondosa strain GF3 isolated from Slovenian forests was cultivated in a liquid substrate based on glucose, with polypeptone as the source of nitrogen and mineral salts, and on a solid substrate based on milled whole maize plant (Zea mays), olive oil and mineral salts. Production of extracellular and intracellular polysaccharides in both substrates was studied. In submerged cultivation, in 28 days 3.65 mg/L of extracellular and 1.30 mg/L intracellular polysaccharide were produced. In solid-state cultivation, in 38 days 3.80 mg/g of extracellular and 0.70 mg/g of intracellular polysaccharide were produced. Polysaccharides were further separated by ion-exchange, and gel and affinity chromatography. The isolated polysaccharides were mainly ß-D-glucanes. Immunostimulatory effects of isolates were tested on induction of cytokine (TNF-alpha, IFN-gamma) synthesis in primary cultures of human mononuclear cells (PBMC) isolated from a buffy coat.

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