Volume 17 Part 1 Article 54
Title: Pro-Oxidative Properties of Medicinal Mushroom Extracts
Authors: L.J.L.D. Van Griensven and Song Wei
Polysaccharide extracts of a large range of higher basidiomycetes fruit bodies were prepared by hot-water extraction and further purified by ethanol precipitation. The resulting solutions contained 0.3–5.0% protein and 0.3-6.1% phenolic compounds. Extracts of all mushrooms activated reactive oxygen species (ROS) in human peripheral blood monocytic cells, in a human monocytic cell line, and in K562 cells. Agaricus bisporus, Coprinus comatus, Phellinus linteus extracts and PSK (Coriolus versicolor) lost all ROS-activating capacity upon removal of the phenolic compounds. Extracts of Agaricus brasiliensis and of Ganoderma lucidum remained active. In A. bisporus, C. comatus, P. linteus and PSK extracts, two different classes of polysaccharides were found: colourless glucan of molecular weight (MW) > 250 kDa that caused no intracellular ROS activation and a light-brown polyphenol–polysaccharide complex of MW 75–200 kDa that was highly active. When the mushroom extracts were tested for their intracellular ROS activation, it was found that a linear relation existed between the concentration of polyphenolic compounds in the preparations and the ROS-activating capacity. All the extracts of a larger group of commercially available medicinal mushroom preparations were found to comply with this tentative rule.Please login to download the PDF for this proceeding.