Volume 17 Part 1 Article 18: Mating Compatibility and Clonality in the Summer Oyster Mushroom, Pleurotus pulmonarius

Volume 17 Part 1 Article 18
Year 2008
Title: Mating Compatibility and Clonality in the Summer Oyster Mushroom, Pleurotus pulmonarius
Author: A.V. Shnyreva

Abstract:

The summer oyster mushroom Pleurotus pulmonarius being a commercially important edible mushroom represents an excellent model organism for population genetics because of its heterothallic type of sexual compatibility and haploid–dikaryotic life cycle which is reproducible under laboratory conditions. Besides, natural populations of the oyster mushrooms can be considered as a source of new germplasm for mushroom breeding. We examined genetic diversity and differentiation among natural populations in central Russia, and somatic and mating compatibility mechanisms. To perform population genetic analysis for Pleurotus we developed several original approaches. First, we used a sampling strategy based on a hierarchical approach. Second, to perform segregation analysis of genetic markers and to test multilocus linkage disequilibrium (associations between loci) in natural populations, we developed a ‘pelletising technique’ based on submerged cultivation of mushroom spore prints in liquid medium. This method allowed analysis of a dikaryotic parent (a functional diploid) and its haploid offspring (individual post-meiotic products, or single-spore isolates) together in the same experiment. Therefore, we were able to perform segregation analysis of genetic markers without tedious crossing. Evidence for hybridisation between dikaryotic and monokaryotic mycelia (di–mon) as an extra source for genetic recombination in the field populations of P. pulmonarius is discussed in the paper. Approaches of population genetics presented in the paper can be applied for analysis of genotypic variability and origin among cultivated mushroom strains of many basidiomycete mushrooms as well as in breeding programs.

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