Volume 17 Part 1 Article 13
Title: Study on the Biological Characteristics of Wild Agaricus bisporus Strains from China
Authors: Zesheng Wang, Jianhua Liao, Hongrong Li, Bo Wang, Meiyuan Chen, Zhenghui Lu and Zhongjie Guo
Field-growing Agaricus strains collected from a meadow of the Tibetan Plateau were confirmed as Chinese wild Agaricus bisporus strains by biological identification and molecular marker analysis. The results showed: 1. The wild strains grew slowly on potato–dextrose–agar medium with weak appressed mycelia, and grew normally in kernel or fermented cottonseed shell substrate. 2. They grew faster than control strain As2796 under lower temperature of 16°C and higher temperature of 32°C, with optimal growing temperature in the range 20–24°C, which was 4°C lower than that of the control strain. 3. In cultivation with manure compost via twice fermentation, the mycelia grew normally in compost and quite slowly in casing soil, and the fruit bodies occurred in lower numbers and later with easier opening and lower production. 4. The fruit body was off-white with a flat and scaled cap, a long stipe and dark gills. The bisporus basidia occupied 70–80% and trisporus basidia 20–30% of the total basidia. 5. Heterokaryotic monospore isolates could fruit in cultivation, and the homokaryotic isolates could cross with those derived from overseas wild A. bisporus strains. 6. The electrophoresis phenotype of isozymes such as esterase etc. belonged to the high production type (H type). 7. The random amplified polymorphic DNA (RAPD) patterns were quite different from those of high production, good quality or hybrid strains, which indicated that the wild strains produce a new kind of RAPD type.Please login to download the PDF for this proceeding.