Volume 17 Part 1 Article 1: Variability in commercial and wild isolates of Agaricus species in Brazil

Volume 17 Part 1 Article 12
Year 2008
Title: Variability in commercial and wild isolates of Agaricus species in Brazil
Authors: F. Silveira Bueno, A. Romão, M. Wach, W. Luiz Araújo and J. Lúcio Azevedo

Abstract:

In Brazil, consistent programs in Agaricus bisporus Peck breeding are not carried out, and lineages used in mushroom production are typically imported and asexually reproduced many times, resulting in significant genetic degeneration. The yield and quality of mushrooms are often affected by various pathogens including fungi, bacteria and viruses, and may often display deformations as a result of genetic degeneration. In order to better understand the variability of Agaricus strains that are cultivated in Brazil, we have collected and characterised by random amplified polymorphic DNA (RAPD) markers the isolates of commercial and wild isolates, to create a local germplasm bank. Forty-eight isolates were obtained including A. bisporus, A. bisporus Portobelo variety, Agaricus bitorquis, Agaricus blazei and two unidentified isolates, in samples drawn from commercial producers, as well as from wild stocks. These isolates were tested by RAPD markers using 6 primers, which yielded 234 polymorphic bands. Genetic similarity tests yielded low levels of polymorphism between commercial A. bisporus isolates originating in the USA and Europe. RAPD analyses proved effective in grouping A. bisporus isolates according to species and geographic origin. Indigenous isolates demonstrated high genetic variability, making them an important source for breeding programs.

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