Volume 12 Part 2 Article 46: Isozyme Analysis of Morchella Species

Volume 12 Part 2 Article 46
Year 1989
Title: Isozyme Analysis of Morchella Species
Authors: R.K. Kulkarni and C.D. Kamerath

Abstract:

Morels (Morchella sp.) are highly savored edible Ascanycetes. The taxonany of this group of fungi is, at present, restricted to morphological features, e.g. the size, shape and color of both ascocarps and ascospores. Smith et al. (1981) described 6 species of Morchella, whereas Delmas and Poitou (1974) delineated 12 species. There is very little information on sexual caiçatibility of single spore isolates (Hervey et al., 1978). Heteiokaryosis has been postulated to occur soon after ascospore germination (Schmidt, 1983). Fruiting under axemc conditions was reported by Ower (1982).

The utility of enzyme electrophoresis in determining genetic relatedness and cell line authentication has been reported for several mushrocm species including Lentinus edodes (Royse et al., 1983), Agaricus brunnescens (Royse and May, 1982) and Pleurotus ostreatus (Kulkarni et al., 1987). Isozyme variation has been used to differentiate between species of Puccmia (Burdon and Marshall, 1981). Enzyme polymorphism can identify genetic markers that are not affected by culture conditions. In addition, since there is a one to one correlation between the electrophoretic phenotype and genotype, the individual’s genotype at a specific gene locus can be determined (May and Royse, 1981).

The objectives of this study were: (1) To identify isozymes that could be clearly resolved by Polyacrylamide gel electrophoresis and that have interprétable enzyme activity banding patterns. (2) Détermine if a given isolate is hcmozygous or heterozygous at a given locus. (3) Identify enzymes that could be used as genetic markers. (4) Evaluate isozyme variation both between and within species with reference to relative mobilities (Rm) of the activity bands. (5) Determine if the presumed genotype grouping by Rm values of isozymes correlates with the current taxonomie classification.

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