Volume 12 Part 2 Article 42
Title: The Effects of Phytohormones on the Mycelial Growth of Calvatia gigantea and Related Species
Authors: J.P. Alexander and B.E. Lippert
Mushrooms of the genus Calvatia produce large, edible fruit bodies (gasterophores) in nature, and produce abundant vegetative mycelium in vitro. The genus has been studied for production of the oncostatic compound Calvacin by Lucas (1960) and Beneke (1963), and germination of the basidiospores by Bulmer & Beneke (1962) and Wilson & Beneke (1966). The nutritional requirements for vegetative mycelium were studied by Sedlmeyr (1960) , and the growth of Calvatia gigantea mycelium on brewery waste discussed by Shannon & Stevenson (1975). To date, Calvatia species have not been induced to fruit in the laboratory, but the size, edibility and tumor retarding potential of the mushrooms make the pursuit desireable.
A number of papers have been generated from several perspectives addressing the effects of growth regulators on growth and differentiation of fungi. Although great variation is reported in response to purportedly comparable treatments, it seems apparent that phytohormones play important roles in fungal growth and perhaps differentiation.
In this preliminary study, we examine the effects of the phytohormones Indole-3-Acetic Acid (IAA), Kinetin and Gibberellic acid (GA3) on the mycelial growth rate of Calvatia gigantea, Calvatia booniana, and Calvatia craniiformis.