Volume 12 Part 2 Article 33: Comparison of Three Methods to Produce Liquid Spawn for Commercial Cultivation of Shiitake

Volume 12 Part 2 Article 33
Year 1989
Title: Comparison of Three Methods to Produce Liquid Spawn for Commercial Cultivation of Shiitake
Author: M. Itävaara

Abstract:

In the coimercial cultivation of shiitake Lentinus edodes (Berk.) Smg, mycelia are ccrmonly grcwn m sawdust or grain and the solid spawn is inoculated into wood logs (Sa Antonio and Hanners, 1983).

In Finland shiitake is cultivated in artificial logs as follows: HD-polyethylene bags are filled with sawdust medium and sterilized and inoculated with the mycelium in liquid form by injection (Pellinen et al., 1987). The mushrocms are cultivated indoors in production rooms where humidity and temperature can be controlled.

The mycelium is currently produced as a static culture, and hcnogenized and diluted before use. With the increase m danand, however, there is need for an ecancmical method of producing large amounts of liquid spawn with high grcwth capacity. With this end in view, static, shaker and fermenter methods of producing the mycelium were conpared. The criterion for good liquid spawn is good adaptation to the sawdust medium. The spawn must be hcmogeneous, consisting of unpelleted mycelia to avoid blocking the injection needles.

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