Volume 12 Part 1 Article 9: Cytological Observations in Agaricus arvensis

Volume 12 Part 1 Article 9
Year 1989
Title: Cytological Observations in Agaricus arvensis
Authors: A.S.M. Sonnenberg and G. Fritsche


The Horse Mushroom, Agaricus arvensis, is a tasty mushroom growing on pastures. Successful cultivation was first reported by Treschow (1944) and later among others by Zadrazil et al. (1973). A. arvensis has a heterothallic type of sexuality with unifactorial incompatibility (Paper & Kaye 1978). The heterothallic nature makes it possible to set up an efficient breeding program. Homokaryons are easily obtained by isolating monospore cultures and strain improvement can be achieved by crossing these infertile monospore cultures (Brian et al. 1981; Fritsche 1979, 1986).

Cytological studies have shown that A. arvensis has the characteristic features of the heterothallic Agaricus species, i.e. multinucleated cells, the absence of clamp connections in heterokaryotic mycelium (Hlavacek 1965; Garibova & Shalashova 1973; Raper & Kaye 1978) and no evidence for nuclear migration in compatible matings (Raper & Kaye 1978). Because of the absence of morphological differences between homo- and heterokaryotic hyphae and because compatible strains do not always interact visibly it is necessary to confirme heterokaryosis by fruiting tests. Occasionally we observed in some strains of A. arvensis.

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