Volume 12 Part 1 Article 3
Title: Fungicide Resistance in Agaricus bisporus I. In Vitro Production and Assessment of Resistance Mutants
Authors: M.P. Challen, K.L. Wilson and T.J. Elliott
The mushroom Agaricus bisporus (Lange) Irribach, is secondarily homothallic (Elliott 1972; Raper, Raper & Miller 1972) with a unifactorial mating type systan (Miller & Kananen 1972; Elliott 1972). Hybridisation methods based on the combination of non-fertile, homokaryotic, single spore cultures, offer better prospects for strain inprovement than traditional selection methods (Elliott & Langton 1981). Due to the difficulties of homokaryon isolation and the recognition of heterokaryons, few commercially successful hybrids have been produced (Fritsche 1983).
An alternative strategy suggested by Elliott (1979) involves using induced resistance to antimetabolites to mark the components within a cross and enable ready detection of heterokaryosis. Resistance markers are easier to identify than the alternative of auxotrophic markers, as they can be positively selected. The generation of resistant mushroon varieties should also improve the prospects for the control of fungal diseases such as Verticillium fungicola (Preuss) Hassebr. var. fungicola during cropping (Elliott & Challen 1983).
Previous attempts to produce resistance markers to fungicides using UV mutagenesis have been partially successful (Elliott & Challen 1985). Cross resistance and infertility among and between the markers generated, has so far prevented corpletion of the proposed breeding strategy (Challen & Elliott 1987).
Further efforts have now extended the range and type of resistance markers available in A. bisporus. We report here on the recovery of additional and novel resistance mutants. The in vitro cross resistance between resistant mutants has been further investigated.Please login to download the PDF for this proceeding.