Volume 12 Part 1 Article 14: Selection and Genetic Analysis of Antibiotic-Resistant Mutant Strains in Agrocybe Aegerita

Volume 12 Part 1 Article 14
Year 1989
Title: Selection and Genetic Analysis of Antibiotic-Resistant Mutant Strains in Agrocybe Aegerita
Authors: J. Labarere, T. Noel and M. Imbernon

Abstract:

Only a few genetic markers are known in the edible mushroom Agrocybe aegerita. Markers previously identified are the genes involved in the tetrapolar incompatibility mechanism (Meinhardt F., Leslie J.F. 1982) and in the differentiation of basidiocarps (Esser et al. 1974; Esser K., Meinhardt F. 1977; Meinhardt F., Esser K. 1981).

In this paper we report the selection of other genetic markers easily identifiable in Agrocybe aegerita. Afterwards, these genetic markers will be used to map the genome of A. aegerita, and to carry out fusion and transformaition of protoplasts. A. aegerita is a suitable organism for genetic studies, because its life cycle, which is entirely controlled under laboratory condi- U-ons, can be completed over a relatively short period: 40 to 60 days, according to the strains considered. Previous studies have shown the sensitivity of sane Basidicmycetes to antibiotics (Anderson J. B. , Cenedese R. 1984). In order to select mutations conferring resistance to antibiotics, we studied the effect of four antibiotics on the mycelial growth of homokaryotic strains of A. aegerita and determined mycelial growth inhibitory concentrations. The four antibiotics used (chloranphenicol, necmycin, parcnonycin and tobramycin) are known to inhibit bacterial protein synthesis. Antibiotic-resistant strains were isolated by spontaneous and U.V. induced mutations. Cross resistance of mutant strains was investigated. Antibiotic-resistance was further characterized by segregation analysis of the resistant phenotype, dominance tests and coiplementation tests.

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