Volume 12 Part 1 Article 11
Title: A Study on Intergeneric Hybridization between Pleurotus sajor-caju and Schizophyllum commune by Protoplast Fusion
Authors: Z.R. Liang and S.T. Chang
In the past decade, several papers on intraspecific and interspecific protoplast fusion in filamentous fungi have been published (Ferenczy, 1984; Kevei et al., 1985; Anne et al., 1985). On the other hand, very few papers have been reported about intergeneric protoplast fusion, especially in higher basidiomycetes, though the intergeneric and even more distant fusions in yeasts have been described (Ferenczy, 1985).
Two fungi, Pleurotus sajor-caju and Schizophyllum commune, have been chosen for this study. They are both distantly related higher basidiomycetes and belong to different orders in classification (Ainsworth, 1971). The main purpose of this study is to approach the subject of intergeneric protoplast fusion in higher basidiomycetes and theoretically to inquire into the possibility of outbreeding in mushroom through protoplast fusion. It is well known that P. sajor-caju is a widely commercially cultivated edible fungus in many parts of the world while S. commune is one of the basidial fungi which has been extensively studied (Schwalb et al., 1978). Both fungi have been chosen because they are easy to handle, grow fast as well as fruit easily under laboratory conditions. Moreover, the morphology of its fruit bodies is clearly different and this is a useful characteristic as far as the experiments and the analysis of results are concerned. The main problem in using protoplast fusion as a breeding tool so far is the difficulty in getting a stable hybrid since the nuclear fusion rarely occurs, especially in more distant fusions. However, there may be an advantage to this problem in higher basidiomycetes since the fruit body develops before the nuclear fusion.Please login to download the PDF for this proceeding.