Volume 10 Part 1 Article 71: Problems in Obtaining Pure Cultures of Cantharellus cibarius

Volume 10 Part 1 Article 71
Year 1979
Title: Problems in Obtaining Pure Cultures of Cantharellus cibarius
Authors: S.P. Schouten and M.H. Waandrager

Abstract:

Cantharellus cibarius Fr. is a mycorrhizal fungus which grows in association with a great number of trees (Trappe, 1962). Doak (1934) demonstrated mycorrhizaformation on Pinus strobus and Pinus taeda by placing pieces of a pure culture on the roots of these trees. Subsequent re-isolation of the fungus did not present any problems but isolation methods were not indicated. Hattula and Gyllenberg (1969), who worked with pure cultures of Cantharellus cibarius,do not record any isolation methods. Only Volz (1966) reported three successful pure cultures, obtained from cap tissue. Several investigators, however, did not succeed in obtaining pure cultures of Cantharellus cibarius (e.g., Modess, 1941, reported his negative results). Basidiospores of mycorrhiza-forming Basidiomycetes do not usually germinate. Germination can be induced or stimulated by several methods. Addition of volatile substances from the mycelium producing the spores (Losel, 1964) or from Ceratocystis fagacearum (Bretz) Hunt (Brown and Merrill, 1973; Morton and French, 1967; Oort, 1974), contact with yeasts such as Rhodotorula rubra (Demme) Lodder (Freis, 1941, 1943, 1966), or addition of isovaleric acid to the medium (Losel, 1966) are all means by which basidiospores have been induced to germination.

Contact with roots of the mycorrhizal host may also stimulate germination. Mycorrhizae were obtained aseptically with spores of Thelephora terrestris Fr. (Marx and Ross, 1970), while Theodorou (1971) succeeded in inoculating pine seeds with spores of Rhizopogon luteolus Fr.

All the methods outlined above were applied to Cantharellus cibarius.

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