Volume 19 Part 1 Article 75: Gene expression patterns of Pleurotus ostreatus under heat stress revealed by genomic and transcriptomic data

Volume 19 Part 1 Article 75
Year 2016
Title: Gene expression patterns of Pleurotus ostreatus under heat stress revealed by genomic and transcriptomic data
Author: Jibin Qu, Xiuming Liu, Mengran Zhao, Chenyang Huang, Tom Hsiang, Jinxia Zhang

Abstract:
We report the genome sequence of a monokaryotic Pleurotus ostreatus strain, CCEF00389_1, which is widely cultivated in China. A 34.9-Mb genome assembly was obtained from approximately 81 million Illumina 100 bp reads, giving rise to over 13,000 predicted genes. RNA-Seq data was obtained from four time points under heat stress conditions, and these data were also used to improve genome assembly and gene prediction. By k-means clustering for expression profiles of 450 selected genes related to heat stress, four different expression patterns were detected. Some protein families show coincident expression trends, suggesting that there are different response for different functions to the high temperature. For example, the expression of chitin synthetases and most transcription factors decreased with time. The expression of heat shock proteins and proteins related to trehalose increased at the beginning of heat stress and peaked at 0.5 hour after stress. There were different expression patterns for different sub-families of glucoside hydrolase.

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