Volume 19 Part 1 Article 68
Title: Effect of Temperature Treatment on Myceliel Growth Rate Distribution Patterns of 164 Self-bred Dikaryons from Lentinula edodes L808
Author: Song Chunyan, Yang Xiaochun, Zhang Dan, Xi Lipin, Zhang Lujun, Yu Hailong, Shang Xiaodong, Tan Qi
A total of 164 self-bred dikaryotic progenies derived from Lentinula edodes strain L808 were selected from 207 isolates based on the formation of myclial clamp connections and, together with the parent stain, were inoculated in glass tubes filled with a substrate consisting of 80% sawdust and 20% wheat bran (moisture content 50%, pH 6.5). After the mycelia had colonized the surface of the substrate, the tubes were subjected to a series of six subsequent periods of 5 days each at different temperatures: 25℃, 30℃, 32℃, 34℃, 25℃(recovery) and 25℃(growth), with each incubation period lasting five days. Mycelial growth rates were recorded in each case. Our data revealed that MGR of the isolates varied considerably during exposure to the poikilothermic regimes and no isolate exhibited the same MGR throughout the entire experimental period, indicating a complex system of gene regulation. Consequently, the MGR under constant temperature and the MGR under poikilothermic regimes must both be taken into account in strain evaluation, especially when the mushroom cultivation period is long and the ambient temperatures during growth are highly variable.