Volume 19 Part 1 Article 65: Association Analysis of Seven Agronomic Traits with SSR Markers in Chinese Cultivation Germplasm of Lentinula edodes

Volume 19 Part 1 Article 65
Year 2016
Title: Association Analysis of Seven Agronomic Traits with SSR Markers in Chinese Cultivation Germplasm of Lentinula edodes
Author: Lujun Zhang, Xiaodong Shang, Chunyan Song, Dan Zhang, Hailong Yu, Qi Tan

Abstract:
Xianggu (Shiitake, Lentinula edodes) is the second largest commercial mushroom species in terms of total world production. China has been the largest producer, consumer and exporter of Xianggu in the world. Xianggu cultivation in China is gradually transformed from small-scale production pattern to large-scale professional production and requires accurate, high-quality cultivars. At this moment, most commercial strains are nor well defined, i.e. strains might have different names but are genetically identical or the other way around. Thirty-nine commercial Xianggu cultivars used recently in China, two wild strains isolated respectively from Sichuan and Shaanxi province in China, and two commercial cultivars from Japan were genotyped using 92 SSR markers, distributed equally on the whole genome. Seven agronomic traits including fruiting temperature (FT), time from inoculating to fruiting (vegetative growth time, VGT), single fruit body weight (SW), cap color (CC), cap diameter (CD), step length (SL) and total yield (TY) were selected for association analysis with the genotype. A total of 445 bands were detected of which 433 bands (97.6%) were polymorphic between two or more strains. The Polymorphism Information Content (PIC) values varied from 0.19 to 0.75, and the mean value was 0.47. The genetic similarity values varied from 0.56 to 1.00, and averaged to 0.76. Sixteen strains can be distinguished from all tested strains, i.e. have a unique genotype. The rest clustered in six groups containing 2 to 9 strains with no differences within each group. Agronomic traits and SSR loci were analysed by a GLM (general linear model) program in TASSEL software. The results showed that the differences between the corresponding agronomic traits of the materials were highly significant (P<=0.01). The present study suggested that the genetic diversity and linkage disequilibrium of the tested Xianggu germplasm were suitable for association analysis. The marker-trait association SSR primers developed in present study provides references for the construction of germplasm for Xianggu and can be used for molecular marker assisted breeding. [wpmem_logged_out]Please login to download the PDF for this proceeding.[/wpmem_logged_out]