Volume 19 Part 1 Article 63
Title: Next Generation Sequencing and Agaricus bisporus breeding
Author: Mark Loftus, Michelle Schultz, Michael Kessler, Mark Wach & Richard Kerrigan
The advent of Next Generation Sequencing (NGS) technology has allowed cost-effective mass sequencing of genomes and transcriptomes in many organisms. Consequently, Plant Breeders are now routinely using NGS data to discover new genes and to find new markers for economically important traits. The purpose of this paper is to present several emerging NGS technologies which have potential to be tools for commercial mushroom breeding. We conclude with a description of how NGS data was used to define powerful markers for a US Patent on a brown mushroom strain.