Volume 19 Part 1 Article 54
Title: Antiproliferative Activity of Protein Extracts from Lignosus tigris (Chon S. Tan) on MCF7 cell line
Author: Shin-Yee Fung, Boon-Hong Kong, Nget-Hong Tan, Chon-Seng Tan and Szu-Ting Ng
Mushrooms have received considerable attention for its bioactive compounds with immunomodulatory and anticancer effects. The aim of this study was to investigate the anticancer activity of the fractions and protein components isolated from cold water extract (CWE) of L. tigris sclerotium in breast cancer (MCF7) cells. Breast cancer is one of the most common cancers affecting women. Conventional chemotherapy is often limited by the development of drug resistance and various side effects throughout the body. CWE fractionation was carried out on a Sephadex G-50 gel and three fractions of high (HMW), medium (MMW) and low molecular weight (LMW) were collected. High molecular weight (HMW) fraction exhibited greater cytotoxicity compared to other fractions on MCF7 cells. Proteins of the CWE-HMW were isolated by ammonium sulphate precipitation. The CWE-HMW protein was further purified using anion exchange chromatography and protein compounds were identified by LC-MS/MS analysis. Ammonium sulphate precipitated proteins from HMW fraction showed high antiproliferative activity against the MCF7 cells with IC50 of 1.17μg/ml. No activity was observed in non-protein fraction of the HMW. Anion exchange chromatographic fractions H1, H2, H3 and H10 showed potent cytotoxicity activity against MCF7 cells with IC50 values of 0.79 ± 0.05 μg/mL, 0.72 ± 0.02 μg/mL, 0.78 ± 0.14 μg/mL and 4.17 ± 0.25 μg/mL, respectively and less cytotoxic against breast normal cell lines. LC-MS/MS analysis revealed that lectin was identified as its major component in fraction H10. Mushroom lectins have been shown to possess potential immunomodulatory, antiproliferative and antitumor activities.