Volume 19 Part 1 Article 31: Varieties in bacterial communities in compost during composting and cultivation

Volume 19 Part 1 Article 31
Year 2016
Title: Varieties in bacterial communities in compost during composting and cultivation
Author: W. de Boer, E. Kuramae, P. Klein Gunnewiek, J.J.P. Baars, H.C. van der Horst, N. Klijn

In the production of substrate for Agaricus bisporus CNC grondstoffen uses straw based horse manure from over 2000 horse stables in Europe. In order to obtain a homogenous substrate, over the years CNC grondstoffen has developed a process in which mixing is an essential part in order to achieve a constant high quality compost. Compost quality is intensely monitored during production by sampling. Among other parameters pH, Ash, Nitrogen and total solids are determined. But how representative is a sample from a tunnel of 4m height*6m wide*30 m long and how homogeneous is compost in a tunnel in reality? Since composting is mainly a decomposition process in which bacteria and fungi play a major role the environmental conditions create the community of microbial decomposers. In other words when we measure the composition of bacterial communities at different locations (horizontal and vertical) in tunnels and during cultivation, differences or shifts in microbial community indicate the variation and/or changes in the system.

In this study the composition of bacteria and fungi in compost used for mushroom cultivation was determined. The substrate has been examined from end phase 2 (end of thermophilic phase before inoculation with Agaricus) till the end of 2nd flush of mushrooms.

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