Volume 19 Part 1 Article 25
Title: Olfactory mechanism of preferable host-Flammulina velutipes selection by the stored food mite Tyrophagus putrescentiae
Author: ShaoXuan Qu, Lin Ma, HuiPing Li and JinDi Song
Tyrophagus putrescentiae is one of the cosmopolitan storage mites, commonly occurring in China, and also is an important pest in mushroom cultivation. Efficacy of chemical pesticides in controlling T. putrescentiae is low due to the fact that the mite can burrow into inner tissues and hide in. Chemosensory genes play very important roles in host location or sex pheromone detection, and therefore they were effectively used in pest control. In our study, odor molecules of the preferred host-Flammulina velutipes were verified by behavioral responses of T. putrescentia using an insect olfactory apparatus and identified with GC-MS. Among these substances, β-Caryophyllene (1000-10000 ppm) has a significant attractive effect on the mite. In order to associate the olfactory related genes with host location or sex pheromone detection, chemosensory proteins were identified for the first time in T. putrescentiae, and their 3D structures were predicted by homology modelling to further understand physiological functions, specifically in host recognition. Based on a docking study, we found that (-) -Alloaromadendrene, 2 -Methylnaphthalene and Cyclopentadecane had high binding affinities to TputCSP1. TputCSP1, which may mediate the host recognition.