Volume 19 Part 1 Article 152: Understanding the nutritional requirements of oyster mushroom (Pleurotus sp.) grown in axenic culture

Volume 19 Part 1 Article 152
Year 2016
Title: Understanding the nutritional requirements of oyster mushroom (Pleurotus sp.) grown in axenic culture
Author: Claudia Carrasco-Cabrera, Tina Bell, Michael A. Kertesz

Abstract:
Nitrogen is an essential element in substrates used for mushroom cultivation and its utilization by fungi needs to be understood to maximise productivity. The use of axenic culture can facilitate our understanding of the biology and physiology of cultivated fungi. Techniques available include agar plate and liquid media cultures.

Spent coffee grounds are becoming popular for mushroom cultivation. It is not well known how biologically efficient this substrate is or if compounds such as caffeine become toxic to fungi. To improve our understanding of caffeine metabolism and assimilation by fungi our hypotheses are: (1) the addition of caffeine will have an effect hyphal growth and, (2) when provided at a certain concentration will prevent fungal growth. In addition, (3) the type of nitrogen available (inorganic or organic) will affect fungal growth and mycelium development differently when added to a defined medium.

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