Volume 19 Part 1 Article 149
Title: De novo assembly of the Pleurotus ostreatus CCEF89 hyphea transcriptome and identification of candidate unigenes and enzymes related to decomposing process
Author: Xue-Jun Wu , Qi An, Yu-Cheng Dai
Lignins are the most abundant high-molecular-mass aromatic compounds, which are complex phenolic polymers that reinforce the walls of certain cells in the vascular tissues of higher plants. Using the genome sequence as a reference, a transcriptome approach has been used to examine the lignocellulolytic enzymes produced by fungi when cultured in a complex biomass. In recent decades, Pleurotus ostreatus (Jacq.) P. Kumm. has attracted particular interest because of its edibility and efficiency in producing lignocellulolytic enzymes. P. ostreatus can be easily cultivated using various agronomic waste products as a substrate, such as wheat, rye and rice straw, sugarcane bagasse, corn cobs, and sawdust. Over the life cycle, the degradation and decomposition of lignocellulose has a significant influence on a fungi’s ability to take advantage of the necessary nutriments. Sequencing has demonstrated that P. ostreatus possesses a large repertoire of genes encoding lignocellulolytic enzymes. We examined the transcriptomes of P. ostreatus grown in these substrates treated with deionized water, a certain concentrate of CuCl2 and guaiacol. We also attempted to integrate the transcriptome data. Since the molecular functions of many of the genes that were differentially regulated under our culture conditions are unknown or are poorly characterized, we focused our analysis on degradation enzymes.