Volume 19 Part 1 Article 148
Title: Individualization of nucleus from mycelium of edible mushrooms using fluorescence dyeing
Author: Se Hun Kim, Rhim Ryoo, Kang-Hyeon Ka and Myung Kil Kim
Tricholoma matsutake, Sarcodon aspratus and Polyozellus multiplex are commercially important edible mushrooms in Korea. However, there are few monokaryons of those fungi since it is not easy to isolate new strains from the basidiospores of them. However, we could get the monokaryons and dikaryons from the badiospores of those species by using PDA (Potato dextrose agar) medium. We could identify one nucleus or two nuclei within each cell by double staining of nuclei and septa. We expect that the monokaryons could be used as a good source for studying cultural characteristics, mating system, analysis of functional genes, and so on.