Volume 19 Part 1 Article 145: Expression analysis of polysaccharide hydrolyzing enzyme genes from Tricholoma matsutake

Volume 19 Part 1 Article 145
Year 2016
Title: Expression analysis of polysaccharide hydrolyzing enzyme genes from Tricholoma matsutake
Author: Hiroki Onuma, Yasuhisa Fukuta, and Norifumi Shirasaka

Abstract:
Tricholoma matsutake ( S. Ito et Imai ) Singer, the most famous edible mushroom in Japan that is an ectomycorrhizal fungus. However, artificial cultivation of the mushroom has not been established in a stable condition. This problem is mycelial growth is very slow. Also, sugars available for growth of T. matsutake are limited (e.g. Glucose, Fructose, Maltose, Trehalose, Cellobiose,Starch). As well as considered the weak of cellulase and hemicellulase that necessary degraded cellulose and hemicellulose. Kusuda et al. reported that productivity of the fungus shows very strong activity of β-glucosidase and the enzyme was purified and characterized (Kusuda et al, 2006). But productivity of cellulases (Endglucanase and Cellobiohydrolase) and glucoamylase of the fungus is very weak. Therefore, the cultivation method similar to other saprophytic mushrooms are to be difficult to artificial cultivation. In the artificial cultivation of T.matsutake, it analysis whether T.matsutake are expressing any carbohydrate degradation system, it is important to search for effective sugars for growth. In this study, it is intended to clarify the carbohydrate degrading enzyme genes i nducible and subjected to expression analysis by Real-time PCR.

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