Volume 19 Part 1 Article 143: Characteristics of Korean Variety ‘Sae-Ah’, Agaricus bisporus, developed by Genetic Pholymorphism Analysis

Volume 19 Part 1 Article 143
Year 2016
Title: Characteristics of Korean Variety ‘Sae-Ah’, Agaricus bisporus, developed by Genetic Pholymorphism Analysis
Author: Kab-Yeul Jang, Youn-Lee Oh, Eun-Sun Kim, Minji Oh, Pyung-Gyun Shin and Won-Sik Kong

Abstract:
The production of mushroom cultivation in Korea is approximately 600 billion won, which is 1.6% of the total Korean agricultural output. Annually, ca. 200,000 tons of mushrooms are harvested in Korea. Agaricus bisporus production in 2014 was 11,000 tons. A. bisporus is occupied the fourth largest production following Lentinula edodes, Pleurotus ostreatus, Flamulina velutipes in Korea. In addition, A. bisporus production value in 2014 was 79 billion won. Production scale is small but turnout is high value-added mushrooms. More than 60% of the mushroom varieties currently being cultivated are the foreign varieties in Korea. Moreover variety development has been suspended since 1980. This study is intended to replace the foreign varieties imported to develop and disseminate indigenous variety. It was also carried out to increase the income of mushroom farms. Most of commercial mushrooms including oyster mushroom are heterotallic and have clamp connection. So It is easy to make a variety by using a single spore crossing method. We were trying to develop hybrid varieties of A. bisporus for improve the efficiency of breeding. First, Parental strains, ASI 1038 and 737, were selected from collected strains. And single spores of 1,000 were isolated from ASI 1038 and 737 individually. A new variety, ‘Sae-Ah’, was made by crossing ASI1038-297 and S737-26, homokaryons selected by genetic analysis. Variety ‘Sae-Ah’ of button mushroom, A. bisporus, has been developed by domestic first crossed line. By using the nucleic acid analysis it has been developed a technique to determine the crossing among homokaryons of A.bisporus. Breeding period in the development of this technology has been reduced to three years in five years. After breeding, this variety was confirmed productivity and stability through cultivation in mushroom farm. Mycelium of ‘Sae-Ah’ on CDA, Compost Dextrose Agar, grew well at 25℃ when it was compared with that of ‘505’. This variety can be started fruiting at 13~15℃ and grew well at 13~20℃. The diameter of mature cap and length of stipe was longer than control, ‘505’. The color of fruiting body was typical white. The mycelial running periods on compost were twenty nine days and the days required from casing until harvest of the first fruiting bodies was fourteen days. Varieties of mushrooms ‘Sae-Ah’ have been growing in 215 of 602 farmers in the shortest period as 35.7% penetration rate and completed contract of ordinary license.

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