Volume 19 Part 1 Article 132: Enhancement of the ergothioneine in shiitake (Lentinula edodes) fruiting bodies through sawdust media with different nitrogen sources

Volume 19 Part 1 Article 132
Year 2016
Title: Enhancement of the ergothioneine in shiitake (Lentinula edodes) fruiting bodies through sawdust media with different nitrogen sources
Author: Ji Heon Park, Se Hun Kim, Rhim Ryoo and Sung Suk Lee

Abstract:
Ergothioneine(ERG) is a naturally occurring amino acid analogue, which has been found in bacteria, plants and animals, cannot be made in human cells. ERG has antioxidant, protectant against radiation and anti-inflammatory properties. The objective was to enhance the ergothioneine content in shiitake (Lentinula edodes) fruiting bodies through the culture of sawdust media with different nitrogen sources. To assess the effect of different nitrogen sources on ergothioneine contents, the fungus was grown in sawdust media with four different nitrogen sources. Nitrogen sources including ammonium nitrate, sodium nitrate, ammonium sulfate and histidine at the concentrations of 0, 0.1, 0.2, 0.3% were used. The contents of ergothioneine in shiitake fruiting bodies were quantified by high performance liquid chromatography (HPLC). As a result, ergothioneine was detected in all samples. The fruit bodies cultivated with 0.2% ammonium sulfate showed the highest ergothioneine content, which is 1.7-fold higher than the control. Our results demonstrate that the cultivation of shiitake mushroom in sawdust medium with suitable ammonium sulfate concentration was effective to enhance ergothioneine contents. There was no significant effect on the enhancement of ergothioneine content in sawdust medium with sodium nitrate and histidine group.

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