Volume 19 Part 1 Article 126
Title: Method with High-Throughput Screening Potential Using E. coli Biosensor Enables Searching for Bioactive Compounds from Fungi
Author: Jenni Tienaho, Tytti Sariala, Riina Muilu-Mäkelä, Jesús Martin, Olga Genilloud, Robert Franzén and Matti Karp
Endophyte fungus strains were isolated and pure cultured from Scots pine root tips grown in organic soil in western Finland. The 130 fungus strains were sequenced (ITS1/ITS4) and they belonged to ten different fungus species. Water extracts of the fungal mycelium were tested for their antioxidant properties with H2O2 scavenging test. Also a new microplate technique was used for the rapid real-time screening of antioxidant and antimicrobial properties from extracts and fractions using a recombinant Escherichia coli DPD2511 and DPD2794 biosensors.