Volume 19 Part 1 Article 124: Free-Radical Scavenging and Metabolic Activities of Cultured Mycelium of Paecilomyces Hepiali Extracts

Volume 19 Part 1 Article 124
Year 2016
Title: Free-Radical Scavenging and Metabolic Activities of Cultured Mycelium of Paecilomyces Hepiali Extracts
Author: Lucia Ungvarská Maľučká, Jarmila Harvanová, Zdenka Bedlovičová, Ján Mojžiš, Martin Pavlík, Miriam Bačkorová and Martin Rajtar

Abstract:
In this paper, current research is focused on testing of the antioxidant activity of the mycelium of Paecilomyces hepiali Chen cultivated on various plant substrates and metabolic activity assays of this mycelial biomass with a qualitative interesting substances. The antioxidant activity of the mycelial biomass was performed by DPPH˙ radical scavenging method for different ethanolic extracts base of a millet substrate (No. 1) and chickpeas substrate (No. 2). The extract No. 1 corresponds to the value of IC50DPPH = 1.73 mg/ml and the ethanol extract of No. 2 value was almost 4.5 times higher, that is IC50DPPH = 7.92 mg/ml. Extracts No.1 and No. 2 were separated by column chromatography to the fractions and determine the chemical structure of substances which formed the most effective fraction of the sample No. 1. The chemical structure of all compounds of most active fraction of sample No.1 was analyzed by 1H, 13C, DEPT, COSY, HSQC and HMBC spectra. Antiproliferative effects of extracts from mycelium of Peacilomyces hepiali Chen (strain of fungus Cs-4, maintained by company Mykoforest Velčice under catalogue number MFTCCB022/022013), cultivated on selected substrates of cereals, were tested on three cancer cell lines, namely HeLa, MCF-7 and Jurkat by MTT assays. We observed differences in antiproliferative effects of extracts among tested cell lines, as well as differences related to dilution of tested extracts, usually the most pronounced at highest tested concentration of 1mg/ml.

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