Volume 19 Part 1 Article 115
Title: Fungal Immunomodulatory Protein from Tiger Milk Mushroom, Lignosus rhinocerotis
Author: Renee Lim Lay Hong, Thandeka Z Gambule, Ng Szu Ting, Tan Chon Seng, Fung Shin Yee,Tan Nget Hong, Ayesha Fatima, Chong Chin Hung, Vigneshraaj Pushparajah
Fungal immunomodulatory proteins (FIPs) are a family of novel proteins purified from edible medicinal mushrooms that possess extensive biological functions including anti-tumor, anti-allergy and immunomodulatory responses. To date FIP from Lignosus rhinocerotis (Tiger Milk Mushroom, TMM) has not yet been reported. A new FIP from TMM (FIP-Lrh) was isolated in a previous study and the protein sequence was provided. An in silico study was carried out to analyse the protein and compare it structurally to other FIP proteins. L. rhinocerotis is an important medicinal mushroom found in Malaysia and it has also been used as a food source. The sclerotium has the most medicinal value and is widely used indigenously as treatment for breast cancer, fever, cough, asthma and food poisoning among others. Biocomputational studies offer powerful new methods to predict molecular structures and understand the complex behavior of living organisms, as well as to analyze vast amount of molecular data to make predictions that guide experimental work. Homology modeling is an important technique to obtain three dimensional (3D) structure of the proteins that have not yet been identified. The goals of molecular docking are the identification of a ligand that binds to a specific receptor binding site and of its preferred, energetically most favorable, binding pose.